WebApr 4, 2016 · The FLAG-RbohD protein was affinity purified and subject to anti-FLAG and anti-pSer39 immuoblot analyses. Numbers indicate arbitrary units of RbohD pS39 … WebEnter the email address you signed up with and we'll email you a reset link.
[PDF] Synergistic Activation of the Arabidopsis ... - Semantic Scholar
WebRBOHD is mainly controlled by Ca (2+) via direct binding to EF-hand motifs and phosphorylation by Ca (2+)-dependent protein kinases. Recent studies have, however, … WebJan 31, 2013 · The weak phosphorylation activity of CPKac5 and 6 on RBOHD and RBOHF was unlikely due to their overall kinase activities . This finding was further substantiated … greater union cinemas canberra
Calcium-dependent protein kinase/NADPH oxidase activation …
WebMay 4, 2015 · Actually, it was shown that phospho-dead mutations of RBOHC at positions Ser318 and Ser322, which correspond to Ser343 and Ser347 in RBOHD, compromise its … Arabidopsis thaliana seeds were stratified at 4 °C for 2 days and sown in soil. Plants were grown in a controlled environmental chamber under the following conditions: 23 °C, 70% relative humidity, a 10-h light/14-h dark photoperiod, and a light intensity of 85 µE/m2/s. N. benthamiana plants were grown in the growth … See more A. thaliana ecotype Columbia (Col-0) was used as the WT. Arabidopsis T-DNA insertion lines in the Col-0 background for pire-1 (SALK_079510) and pire-2 … See more For co-immunoprecipitation in N. benthamiana, pENTR-LTI6b-3xFLAG and pENTR-RBOHD were recombined into pEarlygate104 to generate YFP-tagged fusions. … See more The N-terminus (aa1–376) and the C-terminus (aa756–912) of RBOHD cDNA was PCR amplified and directionally cloned into pENTR/D-TOPO (Invitrogen). The … See more In vitro kinase assays were performed as described in previously25. 0.5 μg of purified HIS-PBL13, HIS-BIK1, and HIS-PBL13ΔC and 2 μg of HIS-RBOHD-C were … See more WebExtracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture. 2024-06-08. Application of data-independent acquisition approach to study the … flip book ideas for moon phases