Nano-glo r hibit lytic detection system
Witryna8 kwi 2024 · 在诱导自噬时，LC3报告蛋白被自噬体捕获并降解。 在加入含有LgBiT蛋白和底物的Nano-Glo® HiBiT Lytic Reagent后，LgBiT与HiBiT标签发生相互作用，重新组成明亮发光的NanoBiT®酶。 在多达7个数量级的范围内，发光信号与HiBiT标记的LC3报告蛋白成正比。 数据解读 自噬报告基因分析的读数是定量的，易于解释。 自噬流的增 … WitrynaThe Nano-Glo® HiBiT Lytic Detection System sensitively quantifies HiBiT-tagged proteins in cell lysates using a simple add-mix-read assay protocol. HiBiT is an 11-amino-acid …
Nano-glo r hibit lytic detection system
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WitrynaNano-Glo® HiBiT Lytic Detection System. Bioluminescent method for detecting the total amount of HiBiT-tagged proteins in the cell. N3030, N3040, N3050. Nano-Glo® HiBiT Blotting System. Luminescent method for rapid detection of HiBiT-tagged proteins on blots—no antibodies needed. N2410. Janelia Fluor® HaloTag® Ligands WitrynaPromega Nano-Glo HiBiT Lytic Detection System, 100ml Accurately quantify Any HiBiT Tagged Protein Expressed in Cells in an antibody free, plate based method. …
WitrynaNano-Glo® HiBiT Lytic Detection System. Maximal fold-response was detected for HIF1α-HiBiT expressed under endogenous regulatory conditions. Endogenous expression not only reduces artifacts related to overexpression but also maintains the proper stoichiometry with endogenous binding partners. WitrynaNano-Glo® HiBiT Blotting System 100ml N2410 Each system contains sufficient components to prepare 100ml of reagent. Includes: • 10ml ®Nano-Glo Blotting Buffer …
Witryna11 sty 2024 · The technology offers flexibility in detection since the type of bead can be designed to recognize a variety of different engineered protein tags or each bead can be coated with specific antibodies (AlphaLISA) against different epitopes on the target protein ( Fig. 3A ). WitrynaUse Nano-Glo® HiBiT Lytic Detection System to assess luminescence for each guide RNA tested. Use unedited cells as negative control for background. Option 2: Use HiBiT Blotting to confirm expression of full-length protein. Take a sample of the edited cells and lyse with preferred buffer. Run samples on a gel, transfer proteins to
WitrynaNano Glo HiBiT Extracellular Detection System Part Numbers: N2420, N2421, N2422 Quantify HiBiT-Tagged Proteins Expressed on the Cell Surface Specific, live-cell detection of extracellular expressed or secreted proteins Simple add-mix-read assay format—no antibodies required Quantitate over 7 logs of linear dynamic range Size …
WitrynaNano-Glo® HiBiT Lytic Detection System Bioluminescent method for detecting the total amount of HiBiT-tagged proteins in the cell. N3030, N3040, N3050 Nano-Glo® HiBiT Blotting System Luminescent method for rapid detection of HiBiT-tagged proteins on blots—no antibodies needed. N2410 Janelia Fluor® HaloTag® Ligands sub zero refrigerator lights not workingWitrynaNano-Glo® HiBiT Lytic Detection System. Bioluminescent method for detecting the total amount of HiBiT-tagged proteins in the cell. N3030, N3040, N3050. Nano-Glo® … sub zero refrigerator pc richardsWitryna안녕하세요. 논문을 보고 cetsa 분석을 셋업하고 있습니다. 실험의 원리는 이해를 하고 있고, 실험의 방법등은... subzero refrigerator models 48 inchWitrynaNano-Glo® HiBiT Lytic Detection System. Bioluminescent method for detecting the total amount of HiBiT-tagged proteins in the cell. N3030, N3040, N3050. Nano-Glo® … sub zero refrigerator light bulb replacementWitryna21 lut 2024 · Nano-Glo HiBit Lytic Dete ction System (Promega Ca t No. N3040) 96-well Filter Plates 0.2um PVDF Hydrophilli c Membrane White Polystyrene (Corning Cat No. 3504) painting goblin townWitryna31 sie 2024 · The amounts of HiBiT-tagged proteins in total cell lysates or in pulldown products were estimated with a Nano-Glo ® HiBiT Lytic Detection System (Promega N3030 ... of remaining HiBiT-fused SARAF proteins under each experimental condition in triplicated wells were assayed by the HiBiT lytic detection system as described … sub zero refrigerator light diffuserWitrynaNano-Glo ® HiBiT Lytic Detection System-中文说明书. Nano-Glo. ®. HiBiT Lytic Detection System-中文说明书. 目录号： N3030, N3040, N3050. 编号：CTM516. sub zero refrigerator making clicking noise