Cstps1

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August undefined, 2024

WebJul 8, 2024 · The proteins encoded by the full-length terpene synthase ( CsTPS1 ) and its alternative splicing isoform ( CsTPS1 - AS ) could catalyze the formation of geraniol when GPP was used as a substrate in vitro , whereas the expression of CsTPS1 - AS was only significantly induced by Colletotrichum gloeosporioides and Neopestalotiopsis sp. infection. Web446 Planta(2024)247:443–457 13 furtherpuriedandsequenced.Theresultingsequencewas named CsTPS1 pro. Thereafter,theCsTPS1 pro sequencewasfusedtoa GUSreportergene.Forthis,368nucleotidesupstreamthe

Genome-wide identification and expression analysis of the …

CsTPS1 has previously been characterized as the key gene encoding the enzyme terpene synthase that catalyzes (+)-valencene biosynthesis (Sharon-Asa et al., 2003). Therefore, we screened a large set of citrus transcription factors for activation or inhibition using the promoter of CsTPS1 and a dual-luciferase assay ( Supplementary Fig. S1 ). WebJan 7, 2016 · Escherichia coli (pRSF-CsTps1 and pAC-Mev/Scidi/Aacl) was similarly cultured, extracted, and analyzed by GC–MS. As a result, only one peak generated, which was determined to be linalool by the MS … bitez turkey reviews

Valencene synthase - Trends in Plant Science

WebDec 1, 2003 · Cstps1 was found to be developmentally regulated: transcript was found to accumulate only towards fruit maturation, corresponding well with the timing of valencene accumulation in fruit. Although citrus fruits are non‐climacteric, valencene accumulation and Cstps1 expression were found to be responsive to ethylene, providing further evidence ... WebDec 5, 2013 · • Citrus volatiles confer protection against endoparasitoid wasps Summary Background Egg-laying animals, such as insects, ensure the survival of their offspring by depositing their eggs in favorable environments. To identify suitable oviposition sites, insects, such as the vinegar fly Drosophila melanogaster, assess a complex range of … WebMar 1, 2007 · Recombinant CsTPS1 showed a K m value of 6.8 μM, a V max of 1.1 × 10 −4 μmol/min and V max /K m of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a K m value of 10.5 μM, ... bitfactor srl

Cloning the Cstps1 gene from Citrus sinensis Team

Terpene synthases in cucumber - Wiley Online Library

WebDec 10, 2014 · The coding sequence of (+)-valencene synthase CsTPS1 ( C. sinensis) was obtained from NCBI GeneEntrez Database (GenBank: AF441124.1 ). The sequence was codon optimized for C. glutamicum by the Java Codon Adaptation Tool (JCat) ( Grote et al., 2005) and the optimized gene was synthesized by GeneArt ® life technologies™ … WebMay 18, 2016 · Valencene synthesis is catalyzed by the terpene synthase CsTPS1, but the transcriptional mechanisms controlling its gene expression are unknown. Here, the AP2/ERF (APETALA2/ethylene response factor) transcription factor, CitAP2.10, is characterized as a regulator of (+)-valencene synthesis. bitez summer garden suites and beach hotelWebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass … bitfactor oy

"WebOther disorders. One common alteration (polymorphism) in the CPS1 gene has been associated with increased risk of circulatory problems in newborns and in individuals who … " - Cstps1

Cstps1

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WebMar 6, 2015 · An enzyme encoding valencene synthase gene (Cstps1) was more abundant in Temple than in Murcott. Valencene accounted for 9.4% of total volatile content in Temple, whereas no valencene was detected in Murcott fruit. Murcott expression of Cstps1 is severely reduced. Conclusion: WebCsTPS1- AS reduced the accumulation of geraniol, and the silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. However, the geraniol content and pathogen resistance of CsTPS1-silenced plants and control plants did not signi cantly di er. Further analysis showed that silencing of CsTPS1- AS led to a ...

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WebCstps1 belonged to the terpene synthase-a subfamily of plant, which was the largest clade of terpene synthases and mainly composed of sesquiterpene synthases. The Cstps1 expression was detected in different organs, with high expression levels in leaf and ﬂower, weak expression in stem and root. Real-time qRT-PCR indicated that Cstps1 WebBiochemical goals • In Citrus sinensis, the Cstps-1 gene codes for the production of the enzyme Citrus Valancene Synthase (CVS). • In vivo, CVS reacts with farnesyl pyrophosphate (FPP) to produce the characteristically fragrant valencene (as well as 5 …

WebDec 15, 2024 · However, CsTPS1 and CsTPS6 were down-regulated with the NaCl treatment and reached the lowest expression level at 24 h. Under H 2 O 2 treatment, … WebJun 1, 2004 · The characterization of the Cstps1 gene now provides researchers with an opportunity to engineer plants for high-level production of valencene, a highly valued flavor and fragrance compound. Adapted …

WebMar 6, 2015 · Two thirds of differentially expressed proteins were identified in the pathways of glycolysis, citric acid cycle, amino acid, sugar and starch metabolism. An enzyme encoding valencene synthase gene ( Cstps1) was … WebTwo recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. …

WebJun 1, 2004 · The corresponding residues within Cstps1 were initially identified by primary sequence alignment, then visual inspection of the relevant sequences overlaid onto the …

WebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. d a starke pty ltd v yard \u0026 anor 2012 sasc 19WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase-deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 (this … bitface incWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. dastards crosswordWebMay 18, 2016 · The expression pattern of CitAP2.10 was positively correlated with (+)-valencene content and CsTPS1 expression. Dual-luciferase assays indicated that … bitez west covinaWebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in … das tavistock institut: auftrag: manipulationWebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in vitro characterization of a monoterpene synthase and its regulatory region that we cloned from Camelina sativa (Camelina). Here, we named this gene as C. sativa terpene synthase 1 … das tatort geheimnis mediathekWebJul 28, 2012 · The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR … das tauflied cassiopeia